alpha300 R - confocal Raman imaging

alpha300 R – Confocal Raman Imaging

 

 

While long recognized as the state-of-the-art imaging system, ongoing development resulting from our innovative spirit keeps the WITec's Raman microscope alpha300 R at the forefront of the technology and sets the benchmark in terms of flexibility, sensitivity, speed and performance. These unique characteristics have established the alpha300 R the preeminent confocal Raman imaging system on the market.

 

 

The flexibility of the alpha300 R series allows the system to adapt to all requirements, combine different imaging techniques and to evolve to meet new or expanded needs.

 

alpha300R motorisiert 095 neu

Key Features

  • Confocal Raman Imaging with unprecedented performance in speed, sensitivity, and resolution
  • Hyperspectral image generation with the information of a complete Raman spectrum at every image pixel
  • Excellent diffraction limited lateral resolution of 200 - 300 nm
  • Outstanding depth resolution ideally suited for 3D image generation and depth profiles
  • Ultra-fast Raman imaging option with only 0.76 milliseconds integration time per spectrum
  • Ultra-high throughput lens-based spectroscopic system for highest sensitivity and best performance in spectral resolution
  • Non-destructive imaging technique: no staining of fixation of the sample required

Application Examples

alpha300R stress in diamond

Confocal Raman image of stress in a diamond film. Color coded from red (compressive strain) to blue (tensile strain).

WITec 3D Raman image pollen honey

3D Raman Image of a pollen in crystalline honey. Image parameters: 150 x 150 x 50 pixels = 1,125,000 Raman spectra, scan area: 50 µm x 50 µm x 50µm, integration time per spectrum: 12.2 milliseconds, total acquisition time: 3h 50 min.

alpha300R 3D semiconductor depth scan

Color-coded Raman depth-scan of a strcutured GaN layer used for semiconductors. Violet: GaN; Green: fluorescence along the edges of the structure; Red: Stress in material.

alpha300R 3D emulsion

3D confocal color-coded Raman image of an emulsion of oil (green), alkane (magenta), and water (blue). 30 µm x 30 µm x 11.5 µm, 150 x 150 x 23 pixels, 517,500 Raman spectra, total acquisition time: 23 min.

Specifications

Raman General Operation Modes

  • Raman spectral imaging: acquisition of a complete Raman spectra at every image pixel
  • Planar (x-y-direction) and depth scans (z-direction) with manual sample positioning
  • Image stacks: 3D confocal Raman imaging
  • Time series
  • Single point Raman spectrum acquisition
  • Single-point depth profiling
  • Fibre-coupled UHTS spectrometer specifically designed for Raman microsopy and applications with low light intensities
  • Confocal Fluorescence Microscopy
  • Bright Field Microscopy

 

Basic Microscope Features

  • Research grade optical microscope with 6x objective turret
  • Video system: video CCD camera
  • LED white-light source for Köhler illumination
  • Manual sample positioning in x- and y-direction
  • Fibre coupling
  • Sample size: 120 mm in x- and y-direction, 25 mm in height

 

 

Raman Optional/Upgradable Operation modes

  • Additional lasers, several wavelenghts eligible
  • Additional UHTS-spectrometers (UV, VIS, NIR)
  • Automated, motorized sample positioning and measuring with piezo-driven scan stages, 25 mm travel range (50 mm travel range optional)
  • Automated confocal Raman imaging (25 x 25 mm²; optional 50 x 50 mm²)
  • Automated multi-area and multi-point measurements
  • Full automation: see apyron
  • Ultrafast Raman imaging (1300 spectra per second) optional available
  • Upgradable for epi-fluorescence applications
  • Adapter for higher samples 
  • TrueSurface for Raman depth profiling
  • Autofocus
  • Dark Field Microscopy, Phase Contrast Microscopy, and DIC optional

 

Ultrahigh-throughput UHTS Spectrometers

  • Various lens-based, excitation optimized spectrometers (UV, VIS or NIR) available, all specifically designed for Raman microsopy and applications with low light intensities
  • Fibre-coupled ultrahigh-throughput optical instruments (up to 70 % transmission)
  • Superior peak shape conservation

 

Computer Interface:

  • WITec software for instrument and measurement control, data evaluation and processing

Extensions & Related Instruments

Literature

  • WITec alpha300 Brochure

    Open PDF
    (7.2 MB)
  • Solutions for High-Resolution Confocal Raman Microscopy

    Open PDF
    (3.3 MB)
Go to literature area

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