Raman analyses can be performed on a variety of samples such as gases, liquids, powders and solids. Only pure metals may cause difficulties due to the fact that they are Raman-inactive.
In general, there is no sample preparation (such as dyeing or dissection) required and Raman spectroscopic measurements can be performed in situ, in vitro, and in vivo.
The maximum applicable sample size for Raman imaging is dependent on the Raman microscope capabilities and the maximum scan range is dependent on the integrated scan stage. Typically, sample areas from 200 x 200 µm² up to 50 x 50 mm² can be investigated. In case of sufficiently transparent sample depth scans in the z-direction are also possible.