alpha300 R – 共聚焦拉曼成像
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一直以来，WITec alpha300 R 拉曼显微系统以其世界前沿的成像技术倍受认可，其灵活、快速、高灵敏度、高性能等特性，使其成为共聚焦拉曼成像市场的引领者。
WITec也始终坚持聚焦创新，不断引进新技术，使得 alpha300 R 的快速拉曼成像技术始终保持领先。
WITec alpha300 R 系列成像系统灵活的模块化设计，能够结合不同的成像技术来满足新的扩展需求，为客户提供、定制不同的解决方案。
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主要特性
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共聚焦拉曼成像，具有前所未有的高速度，高灵敏度和高分辨率
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高光谱图像中每个样品点都能获取一张完整的拉曼光谱
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优异的横向分辨率
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出色的纵向分辨率，非常适于3D成像和深度分析
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超快速拉曼成像，单谱积分时间1毫秒以下
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采用超高通光量的镜头采谱，获得最高的灵敏度和最佳的光谱分辨率
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无损成像技术：无需对样品进行染色或标记
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应用实例
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Confocal Raman image of stress in a diamond film. Color coded from red (compressive strain) to blue (tensile strain). -
3D Raman Image of a pollen in crystalline honey. Image parameters: 150 x 150 x 50 pixels = 1,125,000 Raman spectra, scan area: 50 µm x 50 µm x 50µm, integration time per spectrum: 12.2 milliseconds, total acquisition time: 3h 50 min. -
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Color-coded Raman depth-scan of a strcutured GaN layer used for semiconductors. Violet: GaN; Green: fluorescence along the edges of the structure; Red: Stress in material. -
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3D confocal color-coded Raman image of an emulsion of oil (green), alkane (magenta), and water (blue). 30 µm x 30 µm x 11.5 µm, 150 x 150 x 23 pixels, 517,500 Raman spectra, total acquisition time: 23 min.
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技术规格
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Raman General Operation Modes
- Raman spectral imaging: acquisition of a complete Raman spectra at every image pixel
- Planar (x-y-direction) and depth scans (z-direction) with manual sample positioning
- Image stacks: 3D confocal Raman imaging
- Time series
- Single point Raman spectrum acquisition
- Single-point depth profiling
- Fibre-coupled UHTS spectrometer specifically designed for Raman microsopy and applications with low light intensities
- Confocal Fluorescence Microscopy
- Bright Field Microscopy
Basic Microscope Features
- Research grade optical microscope with 6x objective turret
- Video system: video CCD camera
- LED white-light source for Köhler illumination
- Manual sample positioning in x- and y-direction
- Fibre coupling
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Raman Optional/Upgradable Operation modes
- Additional lasers, several wavelenghts eligible
- Additional UHTS-spectrometers (UV, VIS, NIR)
- Automated, motorized sample positioning and measuring with piezo-driven scan stages
- Automated confocal Raman imaging
- Automated multi-area and multi-point measurements
- Full automation: see apyron
- Ultrafast Raman Imaging, optional available
- Upgradable for epi-fluorescence applications
- Adapter for higher samples
- TrueSurface for Raman depth profiling
- Autofocus
- Dark Field Microscopy, Phase Contrast Microscopy, and DIC optional
Ultrahigh-throughput UHTS Spectrometers
- Various lens-based, excitation optimized spectrometers (UV, VIS or NIR) available, all specifically designed for Raman microsopy and applications with low light intensities
- Fibre-coupled ultrahigh-throughput optical Instruments
- Superior peak shape conservation
Computer Interface:
- WITec software for instrument and measurement control, data evaluation and processing
