alpha300 R - confocal Raman imaging

alpha300 R – 共聚焦拉曼成像

  • 一直以来，WITec alpha300 R 拉曼显微系统以其世界前沿的成像技术倍受认可，其灵活、快速、高灵敏度、高性能等特性，使其成为共聚焦拉曼成像市场的引领者。而WITec也始终坚持聚焦创新，不断引进新技术，使得 alpha300 R 的快速拉曼成像技术始终保持领先。

    WITec alpha300 R 系列成像系统灵活的模块化设计，能够结合不同的成像技术来满足新的扩展需求，为客户提供、定制不同的解决方案。

关键特性

    • 共聚焦拉曼成像，具有前所未有的高速度，高灵敏度和高分辨率

    • 高光谱图像中每个样品点都能获取一张完整的拉曼光谱

    • 优异的横向分辨率

    • 出色的纵向分辨率，非常适于3D成像和深度分析

    • 超快速拉曼成像，单谱积分时间1毫秒以下

    • 采用超高通光量的镜头采谱，获得最高的灵敏度和最佳的光谱分辨率

    • 无损成像技术：无需对样品进行染色或标记

应用案例

  • Confocal Raman image of stress in a diamond film. Color coded from red (compressive strain) to blue (tensile strain).
  • 3D Raman Image of a pollen in crystalline honey. Image parameters: 150 x 150 x 50 pixels = 1,125,000 Raman spectra, scan area: 50 µm x 50 µm x 50µm, integration time per spectrum: 12.2 milliseconds, total acquisition time: 3h 50 min.
    • Color-coded Raman depth-scan of a strcutured GaN layer used for semiconductors. Violet: GaN; Green: fluorescence along the edges of the structure; Red: Stress in material.
    • 3D confocal color-coded Raman image of an emulsion of oil (green), alkane (magenta), and water (blue). 30 µm x 30 µm x 11.5 µm, 150 x 150 x 23 pixels, 517,500 Raman spectra, total acquisition time: 23 min.

关键特性

  • Raman General Operation Modes

    • Raman spectral imaging: acquisition of a complete Raman spectra at every image pixel
    • Planar (x-y-direction) and depth scans (z-direction) with manual sample positioning
    • Image stacks: 3D confocal Raman imaging
    • Time series
    • Single point Raman spectrum acquisition
    • Single-point depth profiling
    • Fibre-coupled UHTS spectrometer specifically designed for Raman microsopy and applications with low light intensities
    • Confocal Fluorescence Microscopy
    • Bright Field Microscopy

    Basic Microscope Features

    • Research grade optical microscope with 6x objective turret
    • Video system: video CCD camera
    • LED white-light source for Köhler illumination
    • Manual sample positioning in x- and y-direction
    • Fibre coupling

  • Raman Optional/Upgradable Operation modes

    • Additional lasers, several wavelenghts eligible
    • Additional UHTS-spectrometers (UV, VIS, NIR)
    • Automated, motorized sample positioning and measuring with piezo-driven scan stages
    • Automated confocal Raman imaging
    • Automated multi-area and multi-point measurements
    • Full automation: see apyron
    • Ultrafast Raman Imaging, optional available
    • Upgradable for epi-fluorescence applications
    • Adapter for higher samples 
    • TrueSurface for Raman depth profiling
    • Autofocus
    • Dark Field Microscopy, Phase Contrast Microscopy, and DIC optional

    Ultrahigh-throughput UHTS Spectrometers

    • Various lens-based, excitation optimized spectrometers (UV, VIS or NIR) available, all specifically designed for Raman microsopy and applications with low light intensities
    • Fibre-coupled ultrahigh-throughput optical Instruments
    • Superior peak shape conservation

    Computer Interface:

    • WITec software for instrument and measurement control, data evaluation and processing

扩展&相关设备

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