December 2017

WITec Suite FIVE Software Now Features Active Focus Stabilization

3D Raman image of cellulose fibers and additives in paper. In order to document the precision of the new focus stabilization, a very long measurement time of 46 hours was chosen.

WITec, industry-leading manufacturer of Raman imaging and correlative microscopy systems, has started shipping its Suite FIVE data acquisition, evaluation and processing software with the new ability to compensate for thermal and mechanical variations during long-term measurements.

Confocal Raman microscopy investigations performed on delicate samples often require lower excitation power and a corresponding increase in acquisition time. Active focus stabilization ensures that the ideal focus between the microscope objective and sample is maintained and signal collection is unaffected while temperature or humidity changes. This is accomplished by an automatic routine that uses a reference point to optimize the Raman signal.

This most recent development in WITec’s ongoing effort to provide results-oriented functionality is a standard and fully-integrated enhancement to the powerful Suite FIVE software package.

October 2017

Notes on the 14th Confocal Raman Imaging Symposium

Winners of the Poster Award Thomas Rabl (left) and Vera Dugandžic with WITec’s CEO Joachim Koenen (right).

In the shadow of the world's tallest church, more than 100 scientists met in Ulm at the end of September for the 14th Confocal Raman Imaging Symposium. The lectures thoroughly documented how modern Raman microscopy contributes to gaining new insights into widely varied scientific questions.

In 11 invited lectures three short presentations, the speakers presented new and interesting applications of Raman microscopy to answer questions from life sciences, materials science, geoscience and environmental science.

Full article

Image with participants

Review Video



August 2017

Top launch at M&M 2017: WITec’s RISE Microscopy with ZEISS Sigma 300 SEM

Top: Combination of WITec Raman microscope and ZEISS Sigma 300 SEM. The Raman microscope is attached through a standard port to the SEM.
Bottom: RISE image of a piece of hematite, showing hematite and goethite in several crystal orientations, that were identified from the Raman spectra.

WITec’s solution for correlative Raman-SEM imaging is now available for ZEISS Sigma 300, a field emission scanning electron microscope (FE-SEM). With this jointly-developed system, WITec and ZEISS have furthered their collaboration to provide a fully-integrated instrument available as an OEM product through ZEISS that features a standard, unmodified vacuum chamber and SEM column along with a complete confocal Raman microscope and spectrometer. It expands the range of choices available to the researcher and incorporates generations of experience in Raman spectroscopic imaging and advanced structural analysis.

RISE stands for Raman Imaging and Scanning Electron microscopy. The seamless combination of the two techniques offers a distinct advantage when investigating samples, improves ease-of-use and accelerates experimental workflow. The research-grade optical microscope capability integral to every WITec microscope also helps researchers survey their sample and quickly locate areas of interest. Both the objective and sample stage required for Raman microscopy are placed within the SEM’s vacuum chamber and can therefore remain under vacuum for all measurements; the sample is simply transferred between the Raman and SEM measuring positions using the stage of ZEISS Sigma 300. The configuration allows the Raman microscope to be attached through a standard port of the SEM. The correlation of data and control of Raman measurements are carried out through WITec’s Suite FIVE software, which features a new operating concept with an intuitive interface, automated components and sophisticated software and data analysis routines.

According to Dr. Olaf Hollricher, Co-founder and Director of R&D at WITec, "Our Raman technology can visualize the distribution of chemical species within a sample, and do it quickly. Combine that with the structural resolution of SEM and you get a properly comprehensive understanding of a sample. It’s a powerful instrument that’s intuitive as well."

ZEISS Sigma 300 provides exceptional resolution, contrast and brightness at a price point accessible to most laboratories and working groups. With its Gemini electron optics, including an Inlens secondary electron detector tailored for high-resolution surface-sensitive imaging, the instrument is inherently flexible and precise. FE-SEMs enable structural characterization of particles, surfaces and nanostructures and the ZEISS Sigma series 4-step automated workflow allows for increased productivity.

WITec’s modular Raman technology allows 3D chemical characterization by combining a high-resolution confocal microscope with a high-throughput Raman spectrometer. Raman imaging, pioneered by WITec, is a label-free and non-destructive technique capable of identifying and imaging the molecular composition of a sample, making it an ideal complement to scanning electron microscopy.

"Comprehensive characterization is essential throughout many scientific fields such as battery research, geology and life sciences. The integration of RISE microscopy in our correlative portfolio aims at delivering cutting edge technology to these and many other areas of research. We are very happy that with WITec we have a partner that shares our ambition to drive scientific advancement," says Dr. Michael Rauscher, Head of Business Sector Materials Sciences at ZEISS Microscopy.

"RISE really fulfills the promise of correlative microscopy," says Dr. Philippe Ayasse, Project Manager for RISE microscopy at WITec. “It gives the user the strengths of Raman and SEM without compromise, all consolidated in one easy to use instrument.”

All the functions of the respective stand-alone SEM and Raman systems are preserved when combined. Switching between measurement modes is accomplished quickly and easily through the software, which also facilitates the transformation of Raman spectroscopic data into an image which can then be overlaid onto the SEM image to produce a RISE image. This correlative approach can greatly benefit researchers in nanotechnology, life sciences, geosciences, pharmaceutics, materials research and many other fields of application.

Product Overview Video:



June 2017

2017 WITec Paper Award for Outstanding Scientific Publications

The Winners
GOLD Paper Award (top): Maria O’Brien and Niall McEvoy
with their workhorse, a WITec confocal Raman microscope.
SILVER Paper Award (middle): Helena Nogueira (right) with the paper’s first author Sara Fateixa (middle) and Tito Trindade in front of their WITec confocal Raman microscope.
BRONZE Paper Award (bottom): Jonas Higl (middle) received the 2017 Paper Award certificate from WITec marketing director Harald Fischer (left). Right: Mika Lindén.

From nearly 60 submissions for the 2017 WITec Paper Award, our jury selected the three best publications. They were written by scientists from Ireland, Portugal and Germany who used WITec correlative confocal Raman microscopes to study transition metal dichalcogenides, textile fibers and cement. These papers show in remarkable detail how information on the chemical and structural composition of a material, obtained through this non-destructive technique, can lead to a more comprehensive understanding of a wide range of materials.


The Gold Paper Award is conferred upon Maria O’Brien from Trinity College in Dublin (Ireland) for mapping low-frequency Raman modes of four transition metal dichalcogenides (TMDCs): MoS2, MoSe2, WS2 and WSe2. Together with Niall McEvoy, Damien Hanlon, Toby Hallam, Jonathan Coleman and Georg Duesberg, she used the Raman modes for in-plane and out-of-plane vibrations whose intensities depend on the thickness and the stacking order of the molecules’ layers. The study has shown that the low-frequency Raman modes of these materials reveal additional information compared to conventional Raman modes. The scientists are convinced: “This study presents a major stepping stone in the fundamental understanding of layered materials as mapping the low-frequency modes allows the quality, symmetry, stacking configuration and layer number of 2D materials to be probed over large areas.” They suggest using low-frequency Raman mapping for the analysis of TMDCs that show no significant changes correlated to layer numbers in the high-frequency regions of their Raman spectra.

The Silver Paper Award is given to Helena Nogueira from the University of Aveiro (Portugal). She and her co-authors Sara Fateixa, Manon Wilhelm and Tito Trindade used three-dimensional Raman imaging and surface enhanced Raman scattering (SERS) to monitor the dyeing process of linen textile fibers with methylene blue. This dye is most commonly used for blue coloring and was applied by various procedures. The scientists also visualized how the silver nanoparticles that give textiles antimicrobial properties are distributed along and within the linen fibers. The authors conclude that regarding textile production “… Raman imaging and SERS are valuable assets that complement or eventually provide unique characterization data.”

The Bronze Paper Award goes to Jonas Higl from the University of Ulm (Germany) for a Raman study on hydrating of cementitious materials. With his colleagues Marcus Köhler and Mika Lindén, he used confocal Raman microscopy to document which structures and molecules are formed during the complex process of hydrating C3S clinker. To the knowledge of the authors this study was the first one published using Raman imaging to study hydrating of cement.

The annual awards recognize outstanding scientific work published the preceding year that employed a WITec device as part of its experimental setup. The evaluation criteria include the significance of the results for the scientific community and the originality of the techniques used.


Winning Publications of the 2017 WITec Paper Award

GOLD: Mapping of low-frequency Raman modes in CVD-grown transition metal dichalcogenides: layer number, stacking orientation and resonant effects. Scientific Reports 2016, 6, 19476.
DOI 10.1038/srep19476.

SILVER: SERS and Raman imaging as a new tool to monitor dyeing on textile fibres. Journal of Raman Spectroscopy 2016, 47, 1239. DOI 10.1002/jrs.4947

BRONZE: Confocal Raman microscopy as a non-destructive tool to study microstructure of hydrating cementitious materials. Cement and Concrete Research 2016, 88, 136.
DOI 10.1016/j.cemconres.2016.07.005


Paper Award 2018

WITec recently announced the 2018 WITec Paper Award competition for research articles published in 2017. Scientists from all fields of application in both academia and industry are invited to submit their publications featuring results acquired with a WITec instrument to The deadline for submissions is January 31st, 2018.

June 2017


1997 - 2017
第一台扫描进场光学显微镜(SNOM)(左)& WITec alpha300拉曼显微镜新家族(右)


自1997年成立至今,在从乌尔姆大学物理系毕业的3位公司创始人的努力下,公司不断发展壮大,目前全球拥有60名员工,除德国乌尔姆总部外,分支机构分设在西班牙、美国、中国、日本和新加坡。正如公司价值观“Focus Innovations”所体现,WITec的成功源于不断引进新技术并坚持以高质量、灵活强大的产品提升客户满意度。

20年来,WITec已经成长为世界知名的共聚焦拉曼成像系统制造商,也充分体现着德国品质的内涵。WITec的第一套设备——扫描近场光学显微镜(SNOM/NSOM)至今还在稳定高效地运转,伊利诺伊大学Urbana-Champaign分校Frederick Seitz材料研究实验室的资深研究员Julio Soares博士表示:“实验室当时购买了WITec的第一套NSOM设备,我们很荣幸成为WITec进一步发展的基石并得以载入WITec公司史册。在我看来,几乎不需要任何技术支持但设备还在持续运转,这本身就是一项很大的成就。

WITec研发总监Olaf Hollricher博士说:“回顾整个拉曼成像历史,WITec的创新和发展让我记忆犹新。过去20年我们带来了巨大的拉曼分析的技术优势,而这些发展惠及从半导体到纺织纤维再到癌细胞等众多材料分析领域。我们一直在推进拉曼成像技术新概念的研发,而我们所获的众多奖项就是对这些研发产品的认可。”

成立之初,WITec拉曼显微镜就能实现快速成像,积分时间在每像素毫秒级别。追溯到90年代末,积分时间曾经是1分钟每个像素,由此WITec客户能够以超乎寻常的速度进行测量。所有WITec拉曼成像系统都可以通过样品的光谱信息生成图像,从而识别样品的化学成份结构。WITec也是第一家将多种显微技术融合到一台设备里的公司,WITec 拉曼-扫描电镜(RISE)联用系统就是这一技术的创新产品,这个系统在当下扫描电镜领域备受瞩目。

WITec总裁Joachim Koenen博士说:“WITec过去20年取得的成就令人欣慰。我们自主研发了许多全新的引以为豪的拉曼成像系统和技术,也很高兴这么多年来能就此跟科学界和工业界的客户分享交流。未来还有很多有待开发和应用的拉曼成像技术理念,我和WITec团队热切地期望继续与我们的客户精诚合作。”