alpha300 Ri - 倒立型共焦点ラマン顕微鏡

alpha300 Ri – Inverted Confocal Raman Imaging

  • Experience Raman Imaging from a new angle.

    倒立型光学系でラマンイメージが取得できます。alpha300Ri は、倒立型光学系を採用し、WITec 標準機であるalpha300R共焦点ラマン顕微鏡の機能をそのまま継承しています。

    特に、ライフサイエンス、バイオ医薬、製薬、鉱物の分野において液体試料や大型試料を観察する場合、alpha300Riの倒立型光学系が威力を発揮します。

     

特長

      • 試料を非破壊、非接触、非染色で観察
      • 高い共焦点性で高分解能3次元観察
      • 光学回析限界まで達する高分解能観察
      • 観察場所を容易に特定し、高い位置再現性
      • 大型試料への対応
      • モジュール構成により簡単にアップグレード
      • 蛍光観察、微分干渉観察(DIC)、位相差観察の機能拡張

アプリケーション例

    • 真核細胞の蛍光顕微鏡像とラマンイメージの合成

      核はDAPIにより染色(青色)

    • Correlative Raman Fluourescence Microscopy: Fluorescence DAPI Staining & Raman Imaging
  • 3D Raman image of pressed banana pulp

    The alpha300 Ri combines the advantages of data acquisition from below with the established merits of 3D confocal Raman imaging which is a powerful and versatile technique that can chemically characterize samples nondestructively and without labeling or other specialized preparation. 

    The image shows a 3D reconstrucion of Raman image-stack acquisition of banana pulp.

    Scan range: 300µm x 200µm x 90µm
    Pixels (Spectra): 450x300x45

  • Pressed banana pulp sample: Starch grains (green) and cell wall components (red)

仕様

  • ラマン一般操作モード

    • Raman spectral imaging: Acquisition of complete hyperspectral Raman imaging data sets while continuously scanning.
    • Planar (x-y-direction) and depth scans (z-direction) with motorized sample positioning
    • Image stacks: 3D confocal Raman imaging 
    • Fast and slow time series
    • Single point Raman spectrum acquisition and depth profiling 
    • Fibre-coupled UHTS spectrometer specifically designed for Raman microsopy and applications with low light intensities
    • Confocal Fluorescence Microscopy 

    基本顕微鏡特長

    • Research grade inverted 
    • Raman spectral imaging: Acquisition of complete hyperspectral Raman imaging data sets while continuously scanning.
    • Planar (x-y-direction) and depth scans (z-direction) with motorized sample positioning
    • Image stacks: 3D confocal Raman imaging   
    • Single point Raman spectrum acquisition and depth profiling
    • Fibre-coupled UHTS spectrometer specifically designed for Raman microsopy and applications with low light intensities
    • Confocal Fluorescence Microscopy 
    • optical microscope with 6x objective turret
    • Video system: Video CCD camera and/or fluorescence camera 
    • LED white-light source for Köhler illumination 
    • Binocular
    • Condensor lens for up to 7 contrasts (e. g. brightfield, DIC, Phase contrast, NAMC, etc.)
    • Sample holder accommodating various standard sample formats (e. g. microscopy slides, Terasakiplates, 35/65 mm dishes, counting chambers)
    • Internal filter block revolver
    • Motorized sample positioning and scanning stage in x- and y-direction, large travel range: 110 mm x 70 mm
  • Raman Optional/Upgradable Operation modes

    • A broad range of lasers and wavelenghts (from UV to IR) eligible
    • Additional UHTS-spectrometers (UV, VIS, NIR)
    • Ultrafast Raman imaging (1300 spectra per second) optional available
    • Upgradable for time correlated single photon counting / fluorescence lifetime imaging (FLIM)
    • Autofocus

    Ultrahigh-throughput WITec UHTS Spectrometers

    • Various lens-based, excitation optimized spectrometers (UV, VIS or NIR) available, all specifically designed for Raman microsopy and applications with low light intensities
    • Fibre-coupled ultrahigh-throughput optical instruments (up to 70 % transmission)
    • Superior peak shape conservation

    Computer Interface:

    • WITec software for instrument and measurement control, data evaluation and processing

Scientists’ Choice Award Winner

  • The WITec alpha300 Ri inverted Raman microscope has been awarded the SelectScience Scientists’ Choice Award 2019. The award is given based on the votes of researchers themselves, who are asked to choose their favorite new laboratory technology released in 2018. The WITec alpha300 Ri was voted the Best New Product in the Spectroscopy category and received excellent reviews on SelectScience.net.

Extensions & Related Instruments

製品カタログ

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