alpha300 R - confocal Raman imaging

alpha300 R – Confocal Raman Imaging

  • While long recognized as the state-of-the-art imaging system, ongoing development resulting from our innovative spirit keeps the WITec's Raman microscope alpha300 R at the forefront of the technology and sets the benchmark in terms of flexibility, sensitivity, speed and performance. These unique characteristics have established the alpha300 R the preeminent confocal Raman imaging system on the market.

    The flexibility of the alpha300 R series allows the system to adapt to all requirements, combine different imaging techniques and to evolve to meet new or expanded needs.

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Key Features

    • Confocal Raman Imaging with unprecedented performance in speed, sensitivity, and resolution
    • Hyperspectral image generation with the information of a complete Raman spectrum at every image pixel
    • Excellent lateral resolution
    • Outstanding depth resolution ideally suited for 3D image generation and depth profiles
    • Ultra-fast Raman imaging option with under one millisecond integration time per spectrum
    • Ultra-high throughput spectroscopic system for highest sensitivity and best performance in spectral resolution
    • Non-destructive imaging technique: no staining of fixation of the sample required

Application Examples

  • alpha300R stress in diamond
    Confocal Raman image of stress in a diamond film. Color coded from red (compressive strain) to blue (tensile strain).
  • WITec 3D Raman image pollen honey
    3D Raman Image of a pollen in crystalline honey. Image parameters: 150 x 150 x 50 pixels = 1,125,000 Raman spectra, scan area: 50 µm x 50 µm x 50µm, integration time per spectrum: 12.2 milliseconds, total acquisition time: 3h 50 min.
    • alpha300R 3D semiconductor depth scan
      Color-coded Raman depth-scan of a strcutured GaN layer used for semiconductors. Violet: GaN; Green: fluorescence along the edges of the structure; Red: Stress in material.
    • alpha300R 3D emulsion
      3D confocal color-coded Raman image of an emulsion of oil (green), alkane (magenta), and water (blue). 30 µm x 30 µm x 11.5 µm, 150 x 150 x 23 pixels, 517,500 Raman spectra, total acquisition time: 23 min.

Specifications

  • Raman General Operation Modes

    • Raman spectral imaging: acquisition of a complete Raman spectra at every image pixel
    • Planar (x-y-direction) and depth scans (z-direction) with manual sample positioning
    • Image stacks: 3D confocal Raman imaging
    • Time series
    • Single point Raman spectrum acquisition
    • Single-point depth profiling
    • Fibre-coupled UHTS spectrometer specifically designed for Raman microsopy and applications with low light intensities
    • Confocal Fluorescence Microscopy
    • Bright Field Microscopy

    Basic Microscope Features

    • Research grade optical microscope with 6x objective turret
    • Video system: video CCD camera
    • LED white-light source for Köhler illumination
    • Manual sample positioning in x- and y-direction
    • Fibre coupling

  • Raman Optional/Upgradable Operation modes

    • Additional lasers, several wavelenghts eligible
    • Additional UHTS-spectrometers (UV, VIS, NIR)
    • Automated, motorized sample positioning and measuring with piezo-driven scan stages
    • Automated confocal Raman imaging
    • Automated multi-area and multi-point measurements
    • Full automation: see apyron
    • Ultrafast Raman imaging, optional available
    • Upgradable for epi-fluorescence applications
    • Adapter for higher samples 
    • TrueSurface for Raman depth profiling
    • Autofocus
    • Dark Field Microscopy, Phase Contrast Microscopy, and DIC optional

    Ultrahigh-throughput UHTS Spectrometers

    • Various lens-based, excitation optimized spectrometers (UV, VIS or NIR) available, all specifically designed for Raman microsopy and applications with low light intensities
    • Fibre-coupled ultrahigh-throughput optical instruments
    • Superior peak shape conservation

    Computer Interface:

    • WITec software for instrument and measurement control, data evaluation and processing

Extensions & Related Instruments

Literature

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