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What is confocal Raman microscopy?

The confocal Raman imaging technique combines Raman spectroscopy with a confocal microscope by acquiring the information of a complete Raman spectrum at every image pixel. Thus the spatial distribution of the chemical components within the sample is detected and imaged. High-resolution confocal Raman microscopes achieve a lateral resolution at the diffraction limit (~ λ/(2NA); typically 250 – 300 nm for 532 nm excitation wavelength and 100x 0.9 NA objective). A confocal microscope setup is furthermore characterized by an excellent depth resolution (below 1 µm) and allows the generation of 3D Raman images and depth profiles.