For confocal Raman imaging a confocal Raman microscope scans the sample point-by-point and line-by-line, and at every image pixel a complete Raman spectrum is acquired. This process is also called hyperspectral imaging. The acquired multi-spectrum files contain the information of thousands to millions of Raman spectra. These files are analyzed with software to generate an image that displays the spatial distribution of the chemical components. By taking a stack of images at different focal positions (in the z-direction), 3D images can be created.